Ka whakapai ake te hua o te waikawa amino na te mea ka whangai i te momo whakaputa me te puna hauota kua whakaitihia ki nga waahanga hanga e hiahiatia ana e te pūtau, ka taea te whakaiti i te pūngao e whakapau ana te pūtau ki te whakahiato waikawa amino me te waiho i te kaha nui ake mo te hua w I roto i ā mātou arotake hangarau ki ngā tipu whakaranu waikawa amino, he maha ngā wā e aromatawaihia ana te Corn Hydrolysate hei whakakapi ā-wā mō ngā pūtake hauota tino pai ake, ā, ko te pātai tuatahi ko te āhua o te pānga o te waikawa waikawa ki te hua motuhake o te Kua whakaaturia e ngā mahi i whakaputaina mō ngā waihanga waihanga mai i te kākano e taea ana e ēnei awa te tautoko i te hanga rauropi e Corynebacterium glutamicum, ā, ka taea e te momo te whakamahi i ngā waihanga waihanga waihanga mai i te kākano o te kākano hei I pūrongotia e tētahi rangahau i runga i te waiora o te waro waro kore-whakakorea he nui ake te hua o te lysine i roto i te taiao hauora i roto i te taiao waihanga, he tohu pai tēnei e nui ake ana te hua o te waiora i te puna waro. Ehara tēnei i te mea ka piki ake te hua o te Corn Hydrolysate i ia tukanga. Ko te tikanga ka taea e te Corn Hydrolysate te whakapai ake i te hanga waikawa amino ina ōrite te tohu hauota ki te momo, ā, ka taurite te toenga o te pāpāho. Ko te uara mahi o te Corn Hydrolysate i roto i te whakaranu waikawa amino kei roto i te kaha ka taea e ia te kawe mai ki te tukatuka hauota, ehara i te kupu whakaari katoa.
Ko te wāhi o ngā amino acid o te Corn Hydrolysate e whakamāngai ana i te hāngā o ngā kaiwhakamāngai, nā te mea he āhua o ngā amino acid ngā kaiwhakamāngai e tukuna ana e te hydrolysate, ā, ko ngā kaiwhakamāngai e whakamāngai ana i te whakamāngai i tēnei wā e whakamāngai ana i ngā amino acid mai i te tīmatanga. Mō tēnei kaiwhakamāngai e whakamāngai ana i te lysine, ko te wāhi o ngā amino acid o te whānau aspartate me te pū o te amino nitrogen katoa e whakamāngai ana i te maha o ngā carbon e rere mai ana ki te lysine i tērā atu ki te biomass, i ngā hua tāwhai rānei. I tēnei whakamātautau i te fermentation i āwhina ai mātou, ko te rōpū e whakamātautau ana i ngā kaiwhakamāngai āhua e rua o te Corn Hydrolysate, ā, he ōrite ngā totohu o te nitrogen, engari he rerekē ngā wāhi o ngā amino acid; ā, ko te kaiwhakamāngai e whakamāngai ana i te wāhi whānui ake o ngā amino acid kua tāwhai, kua whakamāngai ai i te tāwhai o te lysine i ngā whakamātautau. He kōrero kē tēnei, ā, kāore he whānanga o tēnei ki ngā kaiwhakamāngai katoa. He whakaahua noa tēnei i te mātauranga, ā, he mea e whakamārama ana i te wāhi o ngā amino acid o te Corn Hydrolysate, ā, me tāpiri ngā kaihoko i tēnei. Ko ngā mahi kua whakapublikia mō te corn steep liquor hydrolysate i whakamārama ai i te hononga o ēnei amino acid āhua, ā, ko ngā glutamic acid, alanine, me ngā aspartic acid i whakamārama ai i te whakamāngai ake o ngā hua i tēnei rākau, ā, he whakamārama tēnei i te whāinga o te wāhi. Ko te Corn Hydrolysate he uaua ake i te whakamāngai mō te whakamāngai o ngā amino acid i te wā e whakamārama ana i te wāhi, ā, ina whakamāngai ai e te kaiwhakamāngai hei whakamāngai mātua, kāore hei kaiwhakamāngai āhua o te nitrogen.
He mea nui te taumata o te waikura mō te Corn Hydrolysate nā te mea e whakatau ana i te ōritetanga i waenganui i ngā waikawa amino me ngā peptides koreutu, ā, e pā ana taua ōritetanga ki te tere o te waikura e wātea ana ki te momo whakaputa. Ko te Hydrolysate Kukama me te taumata teitei o te waikawa ka tuku i nga waikawa amino koreutu, ka taea te tango tere, i te wa e iti ake ana te taumata o te waikawa ka tuku i nga peptides maha, ka whakahiatohia i te wa poto. Kāore e pai ake te katoa; ko te toenga tika e whakawhirinaki ana ki te momo, te wāhanga whakaranu, me te hua e hiahiatia ana. I roto i ā mātou kōrero hangarau ki ngā rōpū whakaranu, ka kite noa mātou he pai te mahi o te taumata waipiro iti mō te hanga waikawa amino nā te mea e whakarato ana i tētahi puna hauota tere mō te tipu wawe, me tētahi puna puhoi ake mō te wāhanga whakaputa. Ko ngā anga whakamātautau ahumahi mō ngā pūmua konupora waipiro ka whakaurua te taumata waipiro hei tohu matua, ka whangangahia mā ngā tikanga pēnei i te tikanga pH-stat, ā, he taumata waipiro kua tuhia e tuku ana i te kaihoko he huarahi ki te whakataurite i ngā tuku waipiro waip He tino whai hua te Hākinakina Hākinakina mō te hanga waikawa amino ina whakaritea te taumata waikawa me te ōrite, nā te mea koia te tawhā e hono ana i te kai ki te whanonga whakaranu.
Ko ngā whakainga o ngā pūngao he take mātua i te whakamātautau i ngā amino acid, ā, ko te Corn Hydrolysate he wāhanga e whakamārama ana i tēnei, inā kia hanga i te tika, me ngā whakamātautau hāngai. Ko ngā hydrolysate o ngā rākau (lignocellulosic hydrolysates) e whakamāna ana i ngā whakainga pērā i te furfural, te HMF, me te acetic acid, ā, he iti ngā whakamātautau e whakaatu ana i te uaua o ēnei tauira o ngā mikroko, i te whakamātautau i ēnei pūngao i te wā o te whakamātautau. I tēnei whakamātautau i te Corynebacterium glutamicum, i whakamātautau i te corn stover hydrolysate kāore i whakamāngōngōtia, i tūhono mai te tauira ki te furfural i te wā iti, ā, i whakamātautau i te HMF me te acetic acid i ngā haora, ā, he tohu tēnei i te uaua o tēnei tauira, ā, he whānui ngā whāinga o tēnei hydrolysate kāore i whakamāngōngōtia. He whāinga pūmau tēnei mō tēnei tauira, ā, kāore he whāinga whānui kia whakakore ngā whakamātautau kounga. He tohu anō tēnei i te mea me whakamātautau i te kounga o te Corn Hydrolysate i te horopaki o te uaua o te tauira, nā te mea he utu te whakamāngōngōtanga, ā, kāore pea he hāngai pea mēnā e taea ana e te tauira te whakamātautau i tēnei rere. Ko te Corn Hydrolysate he whānui ngā whāinga i te whakamātautau i ngā amino acid inā kia mātauranga tonu te kaihoko i te hydrolysate me te tauira, ā, inā kia whakamātautau ngā whakamātautau kounga i ngā āhua e whakaatu ana i te whāinga o ngā whakainga.
Ko te ōritetanga e akiaki ana i te whakaputa anō i te hua waikawa amino nā te mea kāore e taea te whakahou i te whakapai ake i te hua, ehara i te whakapai ake i te hua ka taea e te tipu te tuku. I roto i ngā arotake a ngā kaiwhakarato, ka tirohia e mātou te hauota katoa, te hauota amino, te taumata waipiro, te haumākū, te pungarehu, me te pūkete tuku puranga, nā te mea ko aua tawhā e whakaahua ana mēnā he pūmau te Hākinakina Hākinakina hei tautoko i I whakahaerehia e tētahi tipu i tautokohia e mātou te waihanga waikawa amino kotahi, ā, i kite i ngā rerekētanga hua kāore i taea e te rōpū te whakamārama; ko te take matua he tuku Hākinakina Hākinakina i tutuki i te whakaritenga hauota katoa engari he rerekē i roto i te hauota amino me te tohatoha I te wā i neke atu te tipu ki tētahi kaiwhakarato me tētahi whānuitanga kua tautuhia mō aua tawhā me ngā raraunga tuku taumata-wāhanga, ka heke te rerekētanga hua, ā, ka taea e te rōpū te arotahi ki te whakapai ake i te taitara hei utu o te whakatau raruraru. Ko te Corn Hydrolysate he pūāhua o te take koiora, nō reira he mea noa kia rerekē, engari me whāiti, kia tuhia hoki te rerekētanga. I roto i tā mātou whare, ka whakamātauria nga raupapa katoa i mua i te tuku, ā, ka whai tonu te tuhinga tuku i te raupapa, ko te putanga whaihua o te māramatanga. He tino whai hua te Hākinakina Hākinakina mō te hanga waikawa amino ina rite te whanonga o te puranga e whai ake nei ki te mea o mua.
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